30DEC

Libyan International Conference for Health Sciences

The First Libyan International Conference for Health Sciences (2024): Open University, Tripoli, Libya
Mediterranean Journal of Pharmacy and Pharmaceutical Sciences
https://ppj.org.ly/article/doi/10.5281/zenodo.10627412

Mediterranean Journal of Pharmacy and Pharmaceutical Sciences

Original article

Comparative composition, antioxidants, and antimicrobial effects of 3- and 7-day fermented seeds of Nigella sativa

Mansurat B. Falana, Muhammad A. Dikwa, Muhammed R. Asinmi, Quadri O. Nurudeen

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Abstract

The significance of fermentation is gaining more relevance due to the need for better preparation of plants, the desire for better plant constituents, and the aim for the preservation of medicinal plants. Hence, this study sets out to determine and compare the pH, proximate composition, chemical constituents, antioxidant, and antimicrobial effects of 3-day and 7-day fermented seeds of Nigella sativa. The fermentation of the seed was done for 3 and 7 days using 2.5% sugar. The pH of the sample declined from 3.5 to 3.45 during the fermentation. The proximate composition (moisture, ash, lipid, protein, and carbohydrates) was higher for the 3-day fermented sample while fiber was higher for the 7-day sample. The phytochemical constituents (tannins, saponins, phenols, alkaloids, flavonoids, phytates, oxalates, terpenoids, steroids, and glycosides) were higher for the 3-day than the 7-day sample. The Gas Chromatography-Mass spectrometry technique showed peaks that were characterized as 17 and 20 constituents on days 3 and 7 of the sample, respectively. The antioxidant capacity (ranging from 35.50% to 82.69% for the 3-day and from 37.50% to 84.99% for the 7-day sample) varies by the increasing concentration (7.81 mg/mL to 1000 mg/mL) of the sample. The diameter of zones of inhibition tested at different concentrations (25 mg/mL, 50 mg/mL, and 100 mg/mL) showed varying degrees of activity against Proteus vulgaris and Staphylococcus aureus. The zones obtained were higher 8-10 mg/mL against Staphylococcus aureus and 10-12 mg/mL against Proteus vulgaris for the 7-day fermented sample while 8.0 to 9.0 mg/mL was obtained for the 3-day fermented against Staphylococcus aureus and 8.0 to 10.0 mg/mL against Proteus vulgaris. Overall, the results of this study indicated that fermentation contributed to enhancing the bioactive components and antioxidant capacity of the fermented seeds of N. sativa thereby supporting the use of fermentation in the production of value-added functional foods.

Keywords

Bioactive components, fermentation, GC-MS, Nigella sativa, Staphylococcus aureus

References

  1. Bakathir HA, Abbas NA (2011) Detection of the antibacterial effect of nigella sativa ground seeds with water. African Journal of Traditional, Complementary and Alternative Medicines. 8 (2): 159-164. doi: 10.4314/ajtcam. v8i2. 63203
  2. Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, Anwar F (2013) A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pacific Journal of Tropical Biomedicine. 3 (5): 337-352. doi: 10.1016/S2221-1691(13)60075-1
  3. Akram KM, Afzal M (2016) Chemical composition of Nigella sativa Linn: part 2 recent advances. Inflammo-pharmacology. 24 (2-3): 67-79.  doi: 10.1007/s10787-016-0262-7
  4. Saleem U, Ahmad B, Rehman K, Mahmood S, Alam M, Erum A (2012) Nephro-protective effect of vitamin C and Nigella sativa oil on gentamicin associated nephrotoxicity in rabbits. Pakistan Journal of Pharmaceutical Sciences. 25 (4): 727-730. PMID: 23009987.
  5. Alemi F, Kwon E, Poole DP, Lieu T, Lyo V, Cattaruzza F, Cevikbas F, Steinhoff M, Nassini R, Materazzi S, Guerrero-Alba R (2013) The TGR5 receptor mediates bile acid-induced itch and analgesia. The Journal of Clinical Investigation. 123 (4): 1513-1530. doi: 10.1172/JCI64551
  6. Akhtar MS, Panwar J, Yun YS (2013) Biogenic synthesis of metallic nanoparticles by plant extracts. ACS Sustainable Chemistry and Engineering. 1 (6): 591-602. doi: 10.1021/sc300118u
  7. Khan A, Chen HC, Tania M, Zhang DZ (2011) Anticancer activities of Nigella sativa (black cumin). African Journal of Traditional, Complementary and Alternative Medicines. 8 (5): 226-233. doi: 10.4314/ajtcam.v8i5S.10
  8. Randhawa MA, Alghamdi MS (2011) Anticancer activity of Nigella sativa (black seed)-a review. The American Journal of Chinese Medicine. 39 (06): 1075-1091. doi: 10.1142/S0192415X1100941X
  9. Gheita TA, Kenawy SA (2012) Effectiveness of Nigella sativa oil in the management of rheumatoid arthritis patients: a placebo-controlled study. Phytotherapy Research. 26 (8): 1246-1248. doi: 10.1002/ptr.3679
  10. Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H (2011) Phytochemical screening and extraction: A review. Internationale Pharmaceutica Sciencia. 1 (1): 98-106. doi: Nil.
  11. Bimakr M, Rahman RA, Taip FS, Ganjloo A, Salleh LM, Selamat J (2011) Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food and Bioproducts Processing. 89 (1): 67-72. doi: 10.1016/j.fbp.2010.03.002
  12. Abdel-Aal EI, Haroon AM, Mofeed J (2015) Successive solvent extraction and GC-MS analysis for the evaluation of the phytochemical constituents of the filamentous green alga Spirogyra longata. Egyptian Journal of Aquatic Research. 41 (3): 233-246. doi: 10.1016/j.ejar.2015.06.001
  13. Frias J, Miranda ML, Doblado R, Vidal-Valverde C (2005) Effect of germination and fermentation on the anti-oxidant vitamin content and antioxidant capacity of Lupinus albus L. var. Multolupa. Food Chemistry. 92:  211-220. doi: 10.1016/j.foodchem.2004.06.049
  14. El-Naggar T, Gómez-Serranillos MP, Palomino OM, Arce C, Carretero ME (2010) Nigella sativa L. seed extract modulates the neurotransmitter amino acids release in cultured neurons in vitro. Journal of Biomedicine and Biotechnology. 2010: 398312. doi: 10.1155/2010/398312
  15. Akhtar MT, Qadir R, Bukhari I, Ashraf RA, Malik Z, Zahoor S, Murtaza MA, Siddique F, Shah SNH, Saadia M (2020) Antidiabetic potential of Nigella sativa L seed oil in alloxan induced diabetic rabbits. Tropical Journal of Pharmaceutical Research. 19 (2): 283-289. doi: 10.4314/tjpr.v19i2.10
  16. Abdulrahman BO, Osibemhe M, Idoko AS (2016) The status of mineral and anti-nutritional composition of raw and fermented seeds of African locust bean (Parkia biglobosa). International Journal of Current Research in Biosciences and Plant Biology. 3 (2): 1-4. doi. 10.20546/ijcrbp.2016.302.001
  17. Burlando B, Palmero S, Cornara L (2019) Nutritional and medicinal properties of underexploited legume trees from West Africa. Critical Reviews in Food Science and Nutrition. 59 (1): 178-188. doi: 10.1080/10408398.2018. 1551776
  18. Steinkraus K (2018) Handbook of indigenous fermented foods, revised and expanded. CRC press. doi. 10.1201/ 9780203752821
  19. Machado CM, Oishi BO, Pandey A, Soccol CR (2004) Kinetics of Gibberella fujikuroi growth and gibberellic acid production by solid-state fermentation in a packed-bed column bioreactor. Biotechnology Progress. 20 (5): 1449-1453. doi: 10.1021/bp049819x
  20. Choi H, Kang B (2003) Anti-aging effect on skin with 9 repetitive steaming and fermenting process herbal composition extract. The Korea Journal of Herbology. 24 (4): 101-106. doi: 10.6116/kjh.2009.24.4.101
  21. Hur SJ, Lee SY, Kim YC, Choi I, Kim GB (2014) Effect of fermentation on the antioxidant activity in plant-based foods. Food Chemistry. 160: 346-356. doi: 10.1016/j.foodchem.2014.03.112
  22. Hernández-Orte P, Ibarz MJ, Cacho J, Ferreira V (2006) Addition of amino acids to grape juice of the Merlot variety: Effect on amino acid uptake and aroma generation during alcoholic fermentation. Food Chemistry. 98 (2): 300-310. doi: 10.1016/j.foodchem.2005.05.073
  23. Guarner F, Schaafsma GJ (1998) Probiotics. International Journal of Food Microbiology. 39 (3): 237-238. doi: 10.1016/S0168-1605(97)00136-0
  24. AOAC (1990) Official methods of analysis. Association of Official Analytical Chemists. 15th ed., Washington D.C., 223-225, 992-995. ISBN: 0935584-42-0.
  25. Odebiyi OO, Sofowora EA (1978) Phytochemical screening of Nigerian medicinal plants II. Lloydia. 41 (3): 234-246. PMID: 672462.
  26. Srinivasan A, Bianchi DW, Huang H, Sehnert AJ, Rava RP (2013) Noninvasive detection of fetal subchromosome abnormalities via deep sequencing of maternal plasma. The American Journal of Human Genetics. 92 (2): 167-176. doi: 10.1016/j.ajhg.2012.12.006
  27. Mensor LL, Menezes FS, Leitão GG, Reis AS, Santos TC, Coube CS, Leitão SG (2001) Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research. 15 (2): 127-130. doi: 10.1002/ptr.687
  28. Daoud A, Malika D, Bakari S, Hfaiedh N, Mnafgui K, Kadri A (2015) Assessment of polyphenol composition, antioxidant and antimicrobial properties of various extracts of date palm pollen (DPP) from two Tunisian cultivars. Arabian Journal of Chemistry. 12 (8): 3075-3086. doi: 10.1016/j.arabjc.2015.07.014
  29. Bhalodia NR, Shukla VJ (2011) Antibacterial and antifungal activities from leaf extracts of Cassia fistula l.: an ethnomedicinal plant. Journal of Advance in Pharmaceutical Technology and Research. 2: 104-109. doi: 10.4103/ 2231-4040.82956
  30. Azmir J, Zaidul IS, Rahman MM, Sharif KM, Mohamed A, Sahena F, Jahurul MH, Ghafoor K, Norulaini NA, Omar AK (2013) Techniques for extraction of bioactive compounds from plant materials: a review. Journal of Food Engineering. 117 (4): 426-436. doi: 10.1016/j.jfoodeng.2013.01.014
  31. Jansirani D, Saradha R, Salomideborani N, Selvapriyadharshini (2014) Comparative evaluation of various extraction methods of curcuminoids from Curcuma longa. Journal of Chemical and Pharmaceutical Sciences. 4: 286-288. Corpus ID: 98319069.
  32. Kushwaha R, Kumar V, Vyas G, Kaur J (2018) Optimization of different variable for eco-friendly extraction of betalains and phytochemicals from beetroot pomace. Waste and Biomass Valorization. 9. 1485-1494. doi: 10.1007/ s12649-017-9953-6
  33. Banerjee J, Singh R, Vijayaraghavan R, MacFarlane D, Patti AF, Arora A (2017) Bioactives from fruit processing wastes: Green approaches to valuable chemicals. Food Chemistry. 225: 10-22. PMID: 28193402.
  34. Bradbury MG, Egan SV, Bradbury JH (1999) Determination of total cyanogens in cassava roots and all forms of cyanogens in cassava products. Journal of the Science of Food and Agriculture. 79 (4): 593-601. doi.10.1002/(SICI) 1097-0010(19990315)97:4
  35. Kocher GS, Pooja (2011) Status of wine production from guava (Psidium guajava L.): A traditional fruit of India. African Journal of Food Science. 5 (16): 851-860. doi: 10.5897/AJFSX11.008
  36. Camu N, De Winter T, Addo S, Takrama J, Bernaer H, De Vuyst L (2008) Fermentation of cocoa beans: influence of microbial activities and polyphenol concentrations on the flavour of chocolate. Science of Food and Agriculture. 88: 2288-2297. doi: 10.1002/jsfa.3349
  37. Kabir Y, Shirakawa H, Komai M (2019) Nutritional composition of the indigenous cultivar of black cumin seeds from Bangladesh. Progress in Nutrition. 21: 428-434. doi: 10.23751/pn.v21i1-S.6556
  38. Liman AA, Egwin P, Vunchi MA, Ayansi C (2012) Lipase activity in fermented oil seeds of Africa Locust Bean, (Parkia Biglobosa), Castor Seeds (Ricinu Communis) and African Oil Bean (Pentaclethra Macrophylla). Nigerian Journal of Basic and Applied Sciences. 18: 136-140. doi: 10.4314/njbas.v18i1.56869
  39. Hur SJ, Lee SY, Kim YC, Choi I, Kim GB (2014) Effect of fermentation on the antioxidant activity in plant-based foods. Food Chemistry. 160: 346-356. doi: 10.1016/j.foodchem.2014.03.112
  40. Thakkar P, Modi HA, Prajapati JB (2015) Isolation, characterization and safety assessment of lactic acid bacterial isolates from fermented food products. International Journal of Current Microbiology and Applied Science. 4 (4): 713-725. doi: Nil.
  41. Davidson PM, Critzer FJ, Taylor TM (2013) Naturally occurring antimicrobials for minimally processed foods. Annual Review of Food Science and Technology. 4: 163-190. doi: 10.1146/annurev-food-030212-182535
  42. Kreyenschmidt J, Ibald R (2012) Modeling shelf life using microbial indicators. Food Preservation Technology Shelf-Life Assessment of Food. 6: 127-168. doi: 10.1201/b11871-7
  43. Burtis M, Bucar F (2000) Antioxidant activity of Nigella sativa essential oil. Phytotherapy Research. 14 (5): 323-328. doi: 10.1002/1099-1573 (200008)14:5
  44. Singh B, Singh JP, Kaur A, Singh N (2017) Phenolic composition and antioxidant potential of grain legume seeds: A review. Food Research International. 101: 1-16. doi: 10.1016/j.foodres.2017.09.026
  45. Dulf FV, Vodnar DC, Socaciu C (2016) Effects of solid-state fermentation with two filamentous fungi on the total phenolic contents, flavonoids, antioxidant activities and lipid fractions of plum fruit (Prunus domestica L.) by-products. Food Chemistry. 209: 27-36. doi: 10.1016/j.foodchem.2016.04.016
  46. Maria John KM, Thiruvengadam M, Enkhtaivan G, Kim DH (2014) Variation in major phenolic compounds and quality potential of CTC black tea elicited by Saccharomyces cercevisiae and its correlation with antioxidant potential. Industrial Crops Production. 55: 289-294. doi: 10.1016/j.indcrop.2014.02.006
  47. Mazid M, Khan TA, Mohammad F (2011) Role of secondary metabolites in defense mechanisms of plants. Biology and Medicine. 3 (2): 232-249. Corpus ID: 13943474.    
  48. Septembre-Malaterre A, Remize F, Poucheret P (2018) Fruits and vegetables, as a source of nutritional compounds and phytochemicals: Changes in bioactive compounds during lactic fermentation. Food Research International. 104: 86-99. doi: 10.1016/j.foodres.2017.09.031
  49. Villarreal-Soto SA, Beaufort S, Bouajila J, Souchard JP, Renard T, Rollan S, Taillandier P (2019) Impact of fermentation conditions on the production of bioactive compounds with anticancer, anti-inflammatory and antioxidant properties in kombucha tea extracts. Process Biochemistry. 83: 44-54. doi: 10.1016/j.procbio.2019. 05.004
  50. Selin, I. Ş, Murat K, Sinem AE (2017) Quantitative analysis of thymoquinone in Nigella Sativa L. (Black Cumin) seeds and commercial seed oils and seed oil capsules from Turkey. Journal of Faculty of Pharmacy of Ankara University. 41 (1): 34-41. doi: 10.1501/Eczfak_0000000593
  51. Erdoğan Ü, Yilmazer M, Erbaş S (2020) Hydrodistillation of Nigella sativa seed and analysis of Thymoquinone with HPLC and GC-MS. Bilge International Journal of Science and Technology Research. 4 (1): 27-30. doi: 10.30516/bilgesci.688845
  52. Ozdemir N, Kantekin-Erdogan MN, Tat T, Tekin A (2018) Effect of black cumin oil on the oxidative stability and sensory characteristics of mayonnaise. Journal of Food Science and Technology. 55 (4): 1562-1568. doi: 10.1007/s 13197-018-3075-4
  53. Shahid W, Durrani R, Iram S, Durrani M, Khan FA (2013) Antibacterial activity in-vitro of medicinal plants. Sky Journal of Microbiology Research. 1 (2): 5-21. doi: Nil.
  54. Sellami M, Ghariani B, Louati H, Miled N, Gargouri Y (2013) Biological activities of extracts of different spices and plants. International Journal of Current Engineering and Technology. 3 (3): 1051-1060. doi: Nil.
  55. Djeussi DE, Noumedem JAK, Seukep JA Fankam AG, Voukeng IK, Tankeo SB, Nkuete AHL, Kuete V (2013) Antibacterial activities of selected edible plants extract against multidrug-resistant gram-negative bacteria. BMC Complementary Medicine and Therapies. 13: 164. 164-172. doi: 10.1186/1472-6882-13-164

Submitted date:
12/05/2023

Reviewed date:
01/24/2024

Accepted date:
01/30/2024

Publication date:
02/06/2024

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