30DEC

Libyan International Conference for Health Sciences

The First Libyan International Conference for Health Sciences (2024): Open University, Tripoli, Libya
Mediterranean Journal of Pharmacy and Pharmaceutical Sciences
https://ppj.org.ly/article/doi/10.5281/zenodo.10728692

Mediterranean Journal of Pharmacy and Pharmaceutical Sciences

Original article

Jobelyn® ameliorates anxiety response and oxido-inflammatory markers induced by tramadol use and discontinuation in rats

Taiwo O. Afe, Akinyinka Alabi, Abayomi M. Ajayi, Ayotunde O. Ale, Oluwatoyin A. Fasesan, Olawale O. Ogunsemi

http://dx.doi.org/10.5281/zenodo.10728692 Mediterr J Pharm Pharm Sci, vol.4, n1, p.93-110, 2024
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Abstract

Jobelyn® is a multi-functional natural dietary supplement made from Sorghum bicolour with very high anti-oxidant and anti-inflammatory capacities. The study investigated the role of Jobelyn® in the attenuation of oxido-inflammatory markers induced by tramadol use, abuse and discontinuation over 17 days in rats. The experimental observational study was carried out using male adult albino rats weighing between 100 and 170 g. The experimental design involved five groups. Rats were randomly divided into groups of five, consisting of group 1 (normal control rats), and group 2 (rats treated with tramadol at 40 mg/kg/day) were administered for 10 days and discontinued for seven days. Group 3 administered incremental doses of tramadol from 40 mg/kg/day to 100 mg/kg/day over 10 days and discontinued for seven days. A similar treatment protocol was administered for group 4 and group 5 but were treated with Jobelyn® at a dose of 200 mg/kg/day at the discontinuation phases for seven days. Behavioral assessments (elevated plus maze model of anxiety and open field model of locomotor activity) and biomarkers of oxido-inflammatory stress were assessed. Tramadol-treated groups had significant anxiety responses and locomotory deficits in comparison to the control group. Tramadol-treated groups had significant elevations of nitrites and malondehyde and reduced enzymatic markers such as catalase, glutathione, reduced glutathione, superoxide dismutase, G-s-transferase, glutamic acid decarboxylase and increased activity of acetylcholinesterase when compared to control group. Administration of Jobelyn® attenuated the responses and ameliorated the oxido-inflammatory biomarkers similar to levels in control group. Tramadol induces oxido-inflammatory stress markers in the prefrontal, striatum and hippocampus in rats. Anxiety and locomotory behavioral actions on tramadol treatment were elevated despite discontinuation for seven days. Thus, Jobelyn® at 200 mg/kg/day ameliorated oxido-inflammatory markers induced by tramadol and decreased anxiety responses in albino rats.

Keywords

Abuse, anxiety response, inflammation, motor activity, oxidative stress, tramadol

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Submitted date:
01/07/2024

Reviewed date:
02/19/2024

Accepted date:
02/28/2024

Publication date:
02/29/2024

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